Role of Estrogen Receptor-alpha in the Regulation of Claudin-6 Expression in Breast Cancer Cells / 한국유방암학회지
Journal of Breast Cancer
;
: 20-27, 2011.
Article
in English
| WPRIM
| ID: wpr-112336
ABSTRACT
PURPOSE:
In our previous studies we showed that upregulating claudin-6 (CLDN6) expression may contribute to preventing breast cancer, and that 17beta-estradiol induces a concentration- and time-related effect on CLDN6 mRNA and protein expression in MCF-7 cells. However, the mechanisms of 17beta-estradiol regulation of CLDN6 are still unclear. We determined the role of estrogen receptors in the regulation of CLDN6 expression in human breast cancer tissues and a cell line.METHODS:
CLDN6, estrogen receptor alpha (ERalpha) and estrogen receptor beta (ERbeta) expression in breast cancer tissues were examined using immunohistochemistry. The human breast cancer cell line, MCF-7, which expresses ERalpha but not ERbeta was used. CLDN6 and ERalpha expression were measured by reverse transcriptase-PCR, Western blotting and immunofluorescent staining. Treatments with propyl pyrazole triol (PPT) and ICI 182, 780 (ICI) were performed.RESULTS:
The results revealed that CLDN6 expression was related to ERalpha in breast cancer tissues (p=0.033). PPT, an ERalpha-selective ligand, upregulated CLDN6 expression at 10-5 mol/L after 24 hours. The effect of PPT on regulating CLDN6 expression in MCF-7 cells was blocked by ICI.CONCLUSION:
These findings suggest that Eralpha reulates CLDN6 expression in breast cancer tissues and that 17beta-estradiol induces CLDN6 expression through an ERalpha pathway in MCF-7 cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pyrazoles
/
Breast
/
Breast Neoplasms
/
RNA, Messenger
/
Immunohistochemistry
/
Receptors, Estrogen
/
Cell Line
/
Blotting, Western
/
Tight Junctions
/
Estrogen Receptor alpha
Limits:
Humans
Language:
English
Journal:
Journal of Breast Cancer
Year:
2011
Type:
Article
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