Production of Interleukin-5, Interleukin-13 and Interferon-gamma in Peripheral Blood CD8+T Cells from Children with Wheezing / 소아알레르기및호흡기학회지
Pediatric Allergy and Respiratory Disease
;
: 228-235, 2008.
Article
in English
| WPRIM
| ID: wpr-112493
ABSTRACT
PURPOSE:
Our objective was to investigate the role of CD8+T cells in pathogenesis of wheezing in children with atopic nature.METHODS:
Twelve atopic wheezers, 8 nonatopic wheezers, 8 disease controls and 8 healthy controls were enrolled in the study. We isolated CD8+ T cells from peripheral blood samples, incubated them for 72 hours either in the absence or presence of phytohemagglutinin (PHA) and compared the concentrations of interleukin (IL)-5, IL-13, and interferon (IFN)-gamma in the cell culture supernatants.RESULTS:
In the atopic wheezer group, the IL-5 concentration was significantly higher after PHA stimulation than after non-stimulated incubation. This difference was not observed in the nonatopic wheezer, disease control and healthy control groups. IL-13 was undetectable in all of the cell culture supernatants. There was no significant difference in the IFN-gamma concentration between the PHA-stimulated and non-stimulated conditions in all 4 groups.CONCLUSION:
The results of this study suggest that CD8+ T cells may play a role in the pathogenesis of wheezing in children with atopic nature through the production of IL-5.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
T-Lymphocytes
/
Respiratory Sounds
/
Interleukins
/
Interferons
/
Interleukin-5
/
Interferon-gamma
/
Interleukin-13
/
Cell Culture Techniques
Limits:
Child
/
Humans
Language:
English
Journal:
Pediatric Allergy and Respiratory Disease
Year:
2008
Type:
Article
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