Organ-specific antigens of Clonorchis sinensis
The Korean Journal of Parasitology
;
: 169-174, 2004.
Article
in English
| WPRIM
| ID: wpr-112668
ABSTRACT
This study was carried out to find out specific proteins from different organs of Clonorchis sinensis. Crude extract, organ-specific and excretory-secretory (ES) proteins were analyzed by immunoblot with infected human sera. The bands of 7- and 17-kDa were main component of intestinal fluid and ES protein and commonly found in all organspecific proteins. The 17-kDa protein was observed from ES antigen, intestinal fluid, eggs and sperms, 26- and 28- kDa proteins were from the uterus, vitellaria, and ovary, and 34-, 37-, 43- and 50-kDa proteins were mainly from the testis and sperms. Serum of mice immunized with sperms reacted to the 50-kDa protein by immunoblotting and immunohistochemical staining showed a positive reaction at the seminal receptacle and seminiferous tubule. The present results show that the 7-kDa protein is a common antigen of every part or organ of C. sinensis, but different organs express their specific antigenic protein bands.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Clonorchis sinensis
/
Mice, Inbred BALB C
/
Antigens, Helminth
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2004
Type:
Article
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