Effects of TGF- beta 3 pretreatment in vitro on the differentiation of rabbit mesenchymal stem cell in vivo / 대한정형외과연구학회지
Journal of Korean Orthopaedic Research Society
;
: 167-176, 2001.
Article
in Korean
| WPRIM
| ID: wpr-113026
ABSTRACT
PURPOSE:
Bone and cartilage were manufactured by using tissue engineering of mesenchymal stem cell (MSC) which can differentiate into variety of cell types. MATERIAL ANDMETHOD:
MSC was isolated and cultured from the rabbit weighing 500g, and it was seeded into PGA mesh and pre-cultured for 1 week with different TGF- beta 3 treated conditions. It was implanted into nude mice and tissues generated were recovered from 1, 2, 3, 4, 8 ,and 12 weeks respectively. Degree of bone and cartilage formation was analyzed with histology and immunohistochemistry assay.RESULT:
Pre-culture condition with TGF- beta 3 treatment showed early start of chondrogenic differentiation, and degree of bone and cartilage formation was promoted as time passed. But both of the cases differentiated into complete bone after 12 weeks.CONCLUSION:
The results show that pretreatment of TGF- beta 3 promotes the differentiation process in vivo condition under the in vivo system where MSC differentiate into bone via cartilage formation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Immunohistochemistry
/
Cartilage
/
Tissue Engineering
/
Mesenchymal Stem Cells
/
Mice, Nude
Limits:
Animals
Language:
Korean
Journal:
Journal of Korean Orthopaedic Research Society
Year:
2001
Type:
Article
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