Apoptotic Effects of 6-Gingerol in LNCaP Human Prostate Cancer Cells
Soonchunhyang Medical Science
;
: 75-79, 2011.
Article
in English
| WPRIM
| ID: wpr-113210
ABSTRACT
OBJECTIVE:
6-Gingerol, one component of ginger (Zingiber officinale) compound, has been known to possess anti-inflammatory, analgesic, anti-emetic, and anti-cancer effects. In this study, the apoptotic ability of 6-gingerol was investigated in human prostate cancer cells.METHODS:
3-(4,5-Dimethylthiazol-2-yl)- 2,5-diphenyl-tetrazolium bromide (MTT) assay, flow cytometry, and western blot analysis were done in LNCaP human prostate cancer cell lines treated with the various doses of 6-gingerol for the different durations of drug exposure.RESULTS:
6-Gingerol in doses ranging from 100 to 300 microM induced dose- and time-dependent inhibition of cell viability in prostate cancer cells by using MTT assay. Maximal inhibition of cell viability was observed at 300 microM of 6-gingerol for 48 hours treatment in LNCaP cells. 6-Gingerol at the dose of 100 microM did not produce any significant change in apoptotic cells in flow cytometry analysis. However, significant increase in sub-G0/G1 phase was observed in cells treated with 200 and 300 microM of 6-gingerol. Any significant cell cycle arrest was not induced by 6-gingerol. In western blotting analysis, expression of caspase-3 was not evident in cells treated with 6-gingerol for 24 hours. However, 48 hours treatment with 6-gingerol altered the expression of caspase-3 in LNCaP cells. Expression of cleaved poly showed the dose-dependent fashion in both 24 hours and 48 hours treatment of 6-gingerol.CONCLUSION:
These observations suggest that 6-gingerol may induce apoptosis in LNCaP human prostate cancer cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Prostate
/
Prostatic Neoplasms
/
Catechols
/
Cell Line
/
Cell Survival
/
Blotting, Western
/
Poly(ADP-ribose) Polymerases
/
Apoptosis
/
Zingiber officinale
/
Caspase 3
Limits:
Humans
Language:
English
Journal:
Soonchunhyang Medical Science
Year:
2011
Type:
Article
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