Characteristics of Mitochondrial Events in Synthetic Bile Acids-induced Apoptosis of Human Osteosarcoma Cells / 체질인류학회지
Korean Journal of Physical Anthropology
;
: 351-362, 2004.
Article
in English
| WPRIM
| ID: wpr-113425
ABSTRACT
ABSTRACT Apoptosis of osteosarcoma cells induced by bile duct derivates, HS-1200 was investigated with relation to mitochodria. HS-1200 induced cytochrome c and Smac/DIABLO release from mitochondria which are major factors related to apoptosis. In these apoptosis processes, release of cytochrome c was not blocked by caspase inhibitor, but release of Smac/DIABLO was blocked. BKA, a kind of PTP (permeablity transition pore) inhibitor, did not block both of them. Interestingly, the alteration of MMP was not observed by means of using JC-1 dye. Although MitoTracker, DiOC-6 and Rhodamine123 were used to confirm previous results, the decrease of MMP was not observed. In order to investigate whether this phenomenon is apoptosis-specific or cell-specific process, genistein was added to cells which usually decreased MMP. After adding genistein, MMP was not decreased, suggesting this phenomenon is cell-specific process. Conclusionally, HS-1200 induced apoptosis of osteosarcoma cells via mitochondria, cytochrome c and Smac/DIABLO were released from mitochondria without decrease of MMP. The release of Smac/DIABLO was dependent of caspase.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bile
/
Bile Ducts
/
Osteosarcoma
/
Apoptosis
/
Genistein
/
Cytochromes c
/
Mitochondria
Limits:
Humans
Language:
English
Journal:
Korean Journal of Physical Anthropology
Year:
2004
Type:
Article
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