Role of LPA and the Hippo pathway on apoptosis in salivary gland epithelial cells
Experimental & Molecular Medicine
;
: e125-2014.
Article
in English
| WPRIM
| ID: wpr-113786
ABSTRACT
Lysophosphatidic acid (LPA) is a bioactive lysophospholipid involved in numerous physiological responses. However, the expression of LPA receptors and the role of the Hippo signaling pathway in epithelial cells have remained elusive. In this experiment, we studied the functional expression of LPA receptors and the associated signaling pathway using reverse transcriptase-PCR, microspectrofluorimetry, western blotting and immunocytochemistry in salivary gland epithelial cells. We found that LPA receptors are functionally expressed and involved in activating the Hippo pathway mediated by YAP/TAZ through Lats/Mob1 and RhoA/ROCK. Upregulation of YAP/TAZ-dependent target genes, including CTGF, ANKRD1 and CYR61, has also been observed in LPA-treated cells. In addition, based on data suggesting that tumor necrosis factor (TNF)-alpha induces cell apoptosis, LPA upregulates TNF-induced caspase-3 and cleaved Poly(ADP-ribose)polymerase (PARP). However, small interfering RNA treatment to Yes-associated protein (YAP) or transcriptional co-activator with a PDZ-binding motif (TAZ) significantly decreased TNF-alpha- and LPA-induced apoptosis, suggesting that YAP and TAZ modulate the apoptotic pathway in salivary epithelial cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphoproteins
/
Salivary Glands
/
Lysophospholipids
/
Signal Transduction
/
Cell Line
/
Gene Expression Regulation
/
Tumor Necrosis Factor-alpha
/
Protein Serine-Threonine Kinases
/
Apoptosis
/
RhoA GTP-Binding Protein
Limits:
Humans
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2014
Type:
Article
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