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Suppression of Tumor Formation and Induction of Natural Killer Cell Activity in BALB/c Nude Mice by Human B7-1 (CD80) Gene Transfer Subcutaneously Injected with Human Hepatocellular Carcinoma Cells (Huh-7) / 대한간학회지
The Korean Journal of Hepatology ; : 124-134, 2003.
Article in Korean | WPRIM | ID: wpr-113811
ABSTRACT
BACKGROUND/

AIMS:

Immunogene therapy is extensively studied for a therapeutic modality of various cancers. This study was conducted to investigate the efficacy of immunogene therapy using the T-cell costimulatory molecule and human B7-1 (CD80, hB7-1) in an in vivo human hepatocellular carcinoma (HCC) model.

METHODS:

The stable HCC cell line expressing hB7-1 gene was established using retroviral vector (Huh-7/hB7-1). Of fourteen BALB/c nude mice, 7 were subcutaneously injected with 2 X 10(6) Huh-7/hB7-1 cells, while the other 7 were injected with 2 X 10(6) Huh-7/mock cells as a control group. After the injection, the mice were observed weekly for three months for subcutaneous tumor formation. Assay for natural killer (NK) cell cytotoxicity and serum IFN-gamma was performed at 1 and 2 weeks after inoculation.

RESULTS:

In BALB/c nude mice inoculated with Huh-7/hB7-1 cells, no tumor growth was observed. BALB/c nude mice inoculated with Huh-7/hB7-1 cells showed significantly increased NK cell activities of splenocytes compared with those with Huh-7/mock cells. Serum IFN-gamma was not measurable at 1 week, but significantly increased at 2 weeks after inoculation to the level of 470 pg/ml in BALB/c nude mice with Huh-7/mock cells and 521 pg/ml in BALB/c nude mice with Huh-7/hB7-1.

CONCLUSIONS:

Our results demonstrate the in vivo anti-tumor immunity and NK cell activation by transfer of hB7-1 gene into human HCC in xenogeneic BALB/c nude mice model. This approach may provide a tool for the development of immunogene therapies against human malignant tumors.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Killer Cells, Natural / Interferon-gamma / Gene Transfer Techniques / B7-1 Antigen / Cytotoxicity, Immunologic / Liver Neoplasms, Experimental / Mice, Inbred BALB C / Mice, Nude / Neoplasm Transplantation Limits: Animals / Humans Language: Korean Journal: The Korean Journal of Hepatology Year: 2003 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Killer Cells, Natural / Interferon-gamma / Gene Transfer Techniques / B7-1 Antigen / Cytotoxicity, Immunologic / Liver Neoplasms, Experimental / Mice, Inbred BALB C / Mice, Nude / Neoplasm Transplantation Limits: Animals / Humans Language: Korean Journal: The Korean Journal of Hepatology Year: 2003 Type: Article