Sulindac-induced Apoptosis without Oligonucleosomal DNA Fragmentation in HT-29 Cells: Its Special References to Mitochondrial Pathway
Journal of the Korean Society of Coloproctology
;
: 191-198, 2004.
Article
in Korean
| WPRIM
| ID: wpr-113836
ABSTRACT
PURPOSE:
This study was undertaken to reveal the molecular mechanism underlying sulindac-induced apoptosis in the human colon cancer cell line HT-29 (mutant p53).METHODS:
Apoptosis was determined by using Hoechst 33342 staining, and translocation of proteins was established by using immunofluorescence, immunoelectron microscopy, and Western blotting after ultra- fractionation.RESULTS:
This type of apoptosis was associated with decreased mitochondrial membrane potential, a translocation of the apoptosis-inducing factor (AIF) to the nucleus, and morphological evidence of nuclear condensation. However, DNA electrophoresis did not elucidate the ladder pattern of DNA fragments. Instead, a pulse-field gel electrophoresis showed that sulindac led to disintegration of nuclear DNA into-high- molecular-weight DNA fragments of about 100~300 kbp.CONCLUSIONS:
Our findings indicate that sulindac induces large-scale DNA fragmentation, suggesting a predominantly AIF-mediated cell-death process, through translocation of the AIF to the nucleus in HT-29 cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA
/
Cell Line
/
Sulindac
/
Blotting, Western
/
Microscopy, Immunoelectron
/
Fluorescent Antibody Technique
/
Apoptosis
/
Colonic Neoplasms
/
HT29 Cells
/
Electrophoresis
Limits:
Humans
Language:
Korean
Journal:
Journal of the Korean Society of Coloproctology
Year:
2004
Type:
Article
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