A Comparative Study of Magnetic-Activated Cell Sorting, Cytotoxicity and Preplating for the Purification of Human Myoblasts
Yonsei Medical Journal
;
: 179-183, 2006.
Article
in English
| WPRIM
| ID: wpr-113994
ABSTRACT
Although cultured myoblast transplantation has been extensively studied as a gene complementation approach to muscular dystrophy treatment, clinical success has still been limited. The inability to adequately isolate and purify myoblasts presents a major limitation to the production of sufficient myoblasts for engrafting purposes. This study attempted to purify myoblasts from primary culture by magnetic-activated cell sorting (MACS), complement-mediated cytotoxicity, and a preplating technique. As a result of positive myoblasts selection by MACS, the average percentage of myoblasts in mixed culture was increased from 30.0% to 41.7%. We observed both myoblast lysis and fibroblast lysis after complement-mediated cytotoxicity. Enrichment of myoblasts in mixed culture was found to increase to 83.1% by using the preplating technique. In addition, higher purification (92.8%) was achieved by following the preplating technique with MACS. Thus, preplating in combination with magnetic-activated cell sorting allows for a rapid and effective isolation of myoblasts from human muscle tissue.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Time Factors
/
Complement System Proteins
/
Immunohistochemistry
/
Cell Differentiation
/
Cell Separation
/
Cells, Cultured
/
Models, Statistical
/
Immunomagnetic Separation
/
Muscle, Skeletal
/
Myoblasts
Type of study:
Prognostic study
/
Risk factors
Limits:
Humans
Language:
English
Journal:
Yonsei Medical Journal
Year:
2006
Type:
Article
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