Restriction fragment length polymorphism analysis of Mycobacterium tuberculosis isolated from Taegu / 대한임상병리학회지
Korean Journal of Clinical Pathology
;
: 581-586, 1999.
Article
in Korean
| WPRIM
| ID: wpr-114661
ABSTRACT
BACKGROUND:
To investigate epidemiology of a specific strain, and evaluate correlation between Mycobacterium tuberculosis restriction fragment length polymorphism (RFLP) and antimicrobial susceptibility, we studied about Mycobacterium tuberculosis RFLP isolated from Taegu area.METHODS:
From Oct. 1997 and Mar. 1999, we isolated 54 strains of M. tuberculosis from the patients visiting Catholic University of Taegu Hyosung, Taegu, Korea. We studied their drug susceptibility and analyzed the Pvu treated RFLP using digoxigenin labeled IS6110 probe.RESULTS:
Fifty-three had more than 6 bands of RFLP and strains with 10 bands were predominant (15 strain). Cluster analysis reveals eleven distinct clusters showing 57.4% of clustered rate (31 strains from A to K) and 35 independent patterns showing 64.8% of the diversity rate at 70% similarity level. Cluster A was the largest group (7 strains) and the next was cluster B (5 strains). Most of the patients with cluster A lived in Taegu city (85.7%) and all of 2 cluster K patients lived in Euisung area. There was no correlation between RFLP pattern and antimicrobial susceptibility, but all two strains of cluster H were resistant to isoniazid. Strains of clustered were also prevalent in the people of middle class.CONCLUSIONS:
Compared to the RFLP analysis in the developed countries, Korea disclosed lower rate of diversity and higher clustered patterns of M. tuberculosis. The clustered strains were also prevalent among the people of middle class.
Full text:
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Index:
WPRIM (Western Pacific)
Main subject:
Tuberculosis
/
Polymorphism, Restriction Fragment Length
/
Developed Countries
/
Epidemiology
/
Digoxigenin
/
Isoniazid
/
Korea
/
Mycobacterium
/
Mycobacterium tuberculosis
Limits:
Humans
Country/Region as subject:
Asia
Language:
Korean
Journal:
Korean Journal of Clinical Pathology
Year:
1999
Type:
Article
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