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Lower growth factor expression in follicular fluid undergone in-vitro fertilization / 대한생식의학회지
Clinical and Experimental Reproductive Medicine ; : 210-215, 2011.
Article in English | WPRIM | ID: wpr-11476
ABSTRACT

OBJECTIVE:

This study was performed to identify whether growth and differentiation factor-9 (GDF-9) and transforming growth factor-beta1 (TGF-beta1) expressions would be lower in the follicular fluid (FF) of those over age 35 who underwent IVF than under age 35.

METHODS:

A total of 24 IVF cycles (20 patients) were included in this study. All of patients were stimulated for IVF by the GnRH short protocol and divided into two groups for analysis, according to their age or =35 group (10 cycles, 9 patients). The expression levels of GDF-9 and TGF-beta1 were determined by western blotting and quantitative enzyme-linked immunosorbent assay.

RESULTS:

The numbers of retrieved oocytes and metaphase II oocytes were significantly lower in the > or =35 group. Lower expression of GDF-9 and TGF-beta1 by western blotting in the > or =35 group were observed as well. The mean GDF-9 and TGF-beta1 levels by enzyme-linked immunosorbent assay were lower in the > or =35 group. The values were 6,850.5+/-928.4 ng/L vs. 3,333.3+/-1,089.2 ng/L of GDF-9 (p<0.05) and 3,844.1+/-571.1 ng/L vs. 2,187.7+/-754.0 ng/L of TGF-beta1 (p<0.05). A negative correlation between GDF-9 and age was observed (r=-0.546, p=0.006).

CONCLUSION:

GDF-9 and TGF-beta1 production from stimulated ovaries during IVF appears to decrease with age.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Oocytes / Ovary / Enzyme-Linked Immunosorbent Assay / Follicular Fluid / Blotting, Western / Gonadotropin-Releasing Hormone / Transforming Growth Factor beta1 / Growth Differentiation Factor 9 / Fertilization / Metaphase Type of study: Prognostic study Limits: Female / Humans Language: English Journal: Clinical and Experimental Reproductive Medicine Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Oocytes / Ovary / Enzyme-Linked Immunosorbent Assay / Follicular Fluid / Blotting, Western / Gonadotropin-Releasing Hormone / Transforming Growth Factor beta1 / Growth Differentiation Factor 9 / Fertilization / Metaphase Type of study: Prognostic study Limits: Female / Humans Language: English Journal: Clinical and Experimental Reproductive Medicine Year: 2011 Type: Article