Differentially expressed genes of Acanthamoeba castellanii during encystation
The Korean Journal of Parasitology
;
: 283-285, 2007.
Article
in English
| WPRIM
| ID: wpr-114844
ABSTRACT
To examine the expressed gene profile during encystation of Acanthamoeba castellanii Castellani, we used differentially expressed gene (DGE) screening by RT-PCR with 20 sets of random primers. From this analysis, we found that approximately 16 genes showed upregulation during encystation. We chose 6 genes, which had relatively higher expression levels, for further investigation. Based on homology search in database, DEG2 showed 55% of similarity with xylose isomerase, DEG9 showed 37% of similarity with Na P-type ATPase, and DEG14 showed 77% of similarity with subtilisin-like serine proteinase. DEG3 and DEG26 were identified as hypothetical proteins and DEG25 exhibited no significant similarity to any known protein. Encystation of Acanthamoeba has been suggested to be a process to resist adverse environmental or nutritional conditions. Further characterization studies of these genes may provide us with more information on the encystation mechanism of Acanthamoeba.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Molecular Sequence Data
/
Protozoan Proteins
/
Up-Regulation
/
Gene Expression Regulation
/
Sequence Alignment
/
Amino Acid Sequence
/
Sequence Homology, Amino Acid
/
Reverse Transcriptase Polymerase Chain Reaction
/
Gene Expression Profiling
/
Acanthamoeba castellanii
Limits:
Animals
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2007
Type:
Article
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