The Effect of Hyperthermia on p-Glycoprotein Expression, Glutathione Content and Glutathione-related Enzyme Activities in Human Renal Cell Carcinoma Cells / 대한비뇨기과학회지
Korean Journal of Urology
; : 168-174, 1999.
Article
in Ko
| WPRIM
| ID: wpr-115022
Responsible library:
WPRO
ABSTRACT
PURPOSE: Resistance to anticancer chemotherapeutic drug remains a major obstacle in cancer chemotherapy. Multidrug-resistance(MDR) gene overexpression and detoxification by glutathione are believed to be involved in adriamycin and cisplatin resistance. We investigated change of p-glycoprotein(MDR gene product) expression, cellular glutathione content and glutathione peroxidase and glutathione transferase activities by hyperthermia to elucidate the synergistic mechanism of hyperthermia with chemotherapeutic agent. MATERIALS AND METHODS: Human renal cell carcinoma cell lines, Caki-1 and A-498 were used. Control temperature was 37OC and hyperthermia of 43OC was applied in 2 and 4 hours durations. P-glycoprotein expression was measured by flowcytometric examination using monoclonal antibody to p-glycoprotein. Glutathione content and activities of glutathione peroxidase and transferase were measured by biochemical methods. RESULTS: Glutathione content and activities of glutathione peroxidase and transferase were not changed by hyperthermia. However, p-glycoprotein expression was reduced by hyperthermia of 43OC. CONCLUSIONS: These results suggest that reduced p-glycoprotein expression by hyperthermia causes increased intracellular accumulation of chemotherapeutic agent by decreasing drug efflux mechanism and plays an important role in synergistic effect with adriamycin and cisplatin cytotoxicities.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Transferases
/
Carcinoma, Renal Cell
/
Doxorubicin
/
Cell Line
/
Cisplatin
/
ATP Binding Cassette Transporter, Subfamily B, Member 1
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Drug Therapy
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Fever
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Glutathione
/
Glutathione Peroxidase
Limits:
Humans
Language:
Ko
Journal:
Korean Journal of Urology
Year:
1999
Type:
Article