Expression of 14-3-3 proteins and Bax in the placenta of preeclampsia / 대한산부인과학회지
Korean Journal of Obstetrics and Gynecology
;
: 858-864, 2007.
Article
in Korean
| WPRIM
| ID: wpr-115043
ABSTRACT
OBJECTIVE:
Purpose of our study was to examine the expression level of 14-3-3 proteins and Bcl-2 family and to estimate the interaction between 14-3-3 proteins and Bcl-2 family in normal and preeclamptic placenta.METHODS:
Placental tissues were sampled from preeclampsia with caesarean delivery (n=5) and normal pregnant women with caesarean delivery (n=5). Western blot and immunoprecipitation related Western blotting were performed on all placental samples. Unpaired Student t-test was used to determine the statistical significance.RESULTS:
Western blot analysis revealed that the expression of Bax and 14-3-3 zeta was significantly greater in the preeclamptic placenta than in the normal placenta. Immunoprecipitation related Western blotting revealed that the interaction between 14-3-3 zeta and Bax was significantly less in the preeclamptic placenta than in the normal placenta.CONCLUSION:
Increased expression of Bax and reduced interaction (between) 14-3-3 zeta and Bax in preeclamptic placenta might influence pathogenesis or sequelae of preeclampsia. Further study is needed to identify the trigger that induces dissociation of Bax from 14-3-3 proteins.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Placenta
/
Pre-Eclampsia
/
Blotting, Western
/
Pregnant Women
/
14-3-3 Proteins
/
Immunoprecipitation
/
Bcl-2-Associated X Protein
Limits:
Female
/
Humans
Language:
Korean
Journal:
Korean Journal of Obstetrics and Gynecology
Year:
2007
Type:
Article
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