Microarray for Genes Associated with Signal Transduction in Diabetic OLETF Keratocytes
Korean Journal of Ophthalmology
;
: 111-119, 2007.
Article
in English
| WPRIM
| ID: wpr-115058
ABSTRACT
PURPOSE:
The purpose of this study was to identify differences in signal transduction gene expression between normal and diabetic keratocytes stimulated with interleukin-1alpha (IL-1alpha) and tumor necrosis factor-alpha (TNF-alpha).METHODS:
Normal and diabetic keratocytes were primarily cultured and treated with 20 ng/ml IL-1alpha and TNF-alpha for 6 h. cDNA was hybridized to an oligonucleotide microarray. Genes identified by the microarray were further evaluated by real-time PCR.RESULTS:
Diabetic keratocytes over-expressed components of the MAPK and Notch pathways, and under-expressed components of the insulin, calcium, and TGF-beta pathways. Cytokine treated diabetic keratocytes differentially expressed components of the TGF-beta and MAPK pathways. After IL-1alpha and TNF-alpha treatment, nine genes were under-expressed, falling in the insulin, TGF-beta, and Toll-like receptor pathways. Real-time PCR showed a significant decrease in the IL-6 and TGF-beta2 genes and a significant increase in the Ppm1a gene.CONCLUSIONS:
There were some differences in gene expression between normal and diabetic keratocytes related to signal transduction pathways, such as the insulin, MAPK, calcium, and TGF-beta pathways. In addition, IL-1alpha and TNF-alpha stimulating the insulin, TGF-beta, and Toll-like receptor signaling pathways may have different effects in diabetic keratocytes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Prolactin
/
DNA
/
Nuclear Proteins
/
Signal Transduction
/
Cells, Cultured
/
Polymerase Chain Reaction
/
Transforming Growth Factor beta
/
Tumor Necrosis Factor-alpha
/
Apoptosis
/
Phosphoric Monoester Hydrolases
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Korean Journal of Ophthalmology
Year:
2007
Type:
Article
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