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Microarray for Genes Associated with Signal Transduction in Diabetic OLETF Keratocytes
Korean Journal of Ophthalmology ; : 111-119, 2007.
Article in English | WPRIM | ID: wpr-115058
ABSTRACT

PURPOSE:

The purpose of this study was to identify differences in signal transduction gene expression between normal and diabetic keratocytes stimulated with interleukin-1alpha (IL-1alpha) and tumor necrosis factor-alpha (TNF-alpha).

METHODS:

Normal and diabetic keratocytes were primarily cultured and treated with 20 ng/ml IL-1alpha and TNF-alpha for 6 h. cDNA was hybridized to an oligonucleotide microarray. Genes identified by the microarray were further evaluated by real-time PCR.

RESULTS:

Diabetic keratocytes over-expressed components of the MAPK and Notch pathways, and under-expressed components of the insulin, calcium, and TGF-beta pathways. Cytokine treated diabetic keratocytes differentially expressed components of the TGF-beta and MAPK pathways. After IL-1alpha and TNF-alpha treatment, nine genes were under-expressed, falling in the insulin, TGF-beta, and Toll-like receptor pathways. Real-time PCR showed a significant decrease in the IL-6 and TGF-beta2 genes and a significant increase in the Ppm1a gene.

CONCLUSIONS:

There were some differences in gene expression between normal and diabetic keratocytes related to signal transduction pathways, such as the insulin, MAPK, calcium, and TGF-beta pathways. In addition, IL-1alpha and TNF-alpha stimulating the insulin, TGF-beta, and Toll-like receptor signaling pathways may have different effects in diabetic keratocytes.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Prolactin / DNA / Nuclear Proteins / Signal Transduction / Cells, Cultured / Polymerase Chain Reaction / Transforming Growth Factor beta / Tumor Necrosis Factor-alpha / Apoptosis / Phosphoric Monoester Hydrolases Type of study: Prognostic study Limits: Animals Language: English Journal: Korean Journal of Ophthalmology Year: 2007 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Prolactin / DNA / Nuclear Proteins / Signal Transduction / Cells, Cultured / Polymerase Chain Reaction / Transforming Growth Factor beta / Tumor Necrosis Factor-alpha / Apoptosis / Phosphoric Monoester Hydrolases Type of study: Prognostic study Limits: Animals Language: English Journal: Korean Journal of Ophthalmology Year: 2007 Type: Article