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Prevalence of tick-borne encephalitis virus in ticks from southern Korea
Journal of Veterinary Science ; : 197-203, 2010.
Article in English | WPRIM | ID: wpr-115096
ABSTRACT
The prevalence of tick-borne encephalitis virus (TBEV) in southern Korea was determined by collecting ticks using tick drags. A total of 4,077 of 6,788 ticks collected were pooled (649 pools) according to collection site, species, and developmental stage and assayed for TBEV. The TBEV protein E and NS5 gene fragments were detected using RT-nested PCR in six pools of nymphs collected from Jeju Island (2,491 ticks). The minimum field detection rates for TBEV were 0.17% and 0.14% for Haemaphysalis longicornis and Haemayphysalis. flava nymphs, respectively. The 252 bp NS5 and 477 bp protein E gene amplicons were sequenced. Phylogenetic analysis showed that the NS5 and protein E genes of the Jeju strain were clustered with Western subtype (98.0% and 99.4% identity, respectively). The Western subtype of TBEV is endemic in Korea, including Jeju Island. The study of vector and zoonotic host susceptibility to TBEV is required to better understand its potential impact on public health.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Arachnid Vectors / Phylogeny / Ticks / Molecular Sequence Data / Base Sequence / Viral Envelope Proteins / Prevalence / Sequence Analysis, DNA / DNA Primers / Reverse Transcriptase Polymerase Chain Reaction Type of study: Prevalence study / Risk factors Limits: Animals Country/Region as subject: Asia Language: English Journal: Journal of Veterinary Science Year: 2010 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Arachnid Vectors / Phylogeny / Ticks / Molecular Sequence Data / Base Sequence / Viral Envelope Proteins / Prevalence / Sequence Analysis, DNA / DNA Primers / Reverse Transcriptase Polymerase Chain Reaction Type of study: Prevalence study / Risk factors Limits: Animals Country/Region as subject: Asia Language: English Journal: Journal of Veterinary Science Year: 2010 Type: Article