Co-Expression of Cox-2, C-Met and beta-catenin in Cells Forming Invasive front of Gallbladder Cancer / Journal of the Korean Cancer Association, 대한암학회지
Cancer Research and Treatment
;
: 171-176, 2005.
Article
in English
| WPRIM
| ID: wpr-115166
ABSTRACT
PURPOSE:
Gallbladder cancer is a malignancy with poor prognosis, predominantly resulting from invasion and metastasis. Our previous studies have demonstrated that prostaglandin E2 (PGE2), generated by cyclooxygenase 2 (Cox-2), transactivates epidermal growth factor receptor (EGFR), c-Met and beta-catenin; thus, enhancing colon cancer cell growth and invasiveness in vitro. To determine whether these findings are applicable to clinical conditions, we examined the expression and cellular localization/co-localization of Cox-2, c-Met, beta-catenin, EGFR and c-erbB2 in gallbladder cancer. MATERIALS ANDMETHODS:
Thirty-five specimens of invasive gallbladder cancer, 8 in situ carcinoma and 7 adenoma specimens were immunostained with specific antibodies against Cox-2, c-Met, beta-catenin, EGFR and c-erbB2. The cellular distribution, localization and co- localization were examined, and the signal intensities quantified in a) the central area of gallbladder cancer and b) cancer cells forming the invasive front.RESULTS:
Cox-2, c-Met, beta-catenin, c-erbB2 and EGFR were over-expressed in 80, 74, 71, 62 and 11% of invasive gallbladder cancers, respectively. beta-catenin was expressed in 80% of non-malignant specimens, exclusively in the cell membrane, while the cancer specimens showed cytoplasmic and/or nuclear staining. Significantly higher Cox-2, c-Met and beta-catenin expressions were present in cancer cells of the invasive front than in the tumor central areas (p<0.001), and these expressions were significantly (p=0.01) associated with the invasion depth. Co- expressions of Cox-2, c-Met, beta-catenin and c-erbB2 were present in 42% of the specimens in cancer cells forming the invasive front.CONCLUSION:
The overexpressions, and often co-localizations, of Cox-2, c-Met and beta-catenin in cancer cells forming the invasive front indicate their local interactions and important roles in invasion.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Prognosis
/
Immunohistochemistry
/
Dinoprostone
/
Adenoma
/
Cell Membrane
/
Colonic Neoplasms
/
Cytoplasm
/
Cyclooxygenase 2
/
Beta Catenin
/
ErbB Receptors
Type of study:
Prognostic study
Language:
English
Journal:
Cancer Research and Treatment
Year:
2005
Type:
Article
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