Antitumor Effects of Arsenic Trioxide on Neuroblastoma / 대한소아혈액종양학회지
Korean Journal of Pediatric Hematology-Oncology
;
: 64-71, 2003.
Article
in Korean
| WPRIM
| ID: wpr-115290
ABSTRACT
PURPOSE:
We aimed to study the feasibility of arsenic trioxide as a treatment of neuroblastoma which has the ability to differentiate into nonmalignant cells like acute promyelocytic leukemia.METHODS:
To determine the effects of arsenic trioxide in various concentrations and exposure time on the survivial of neuroblastoma cell lines, SH-SY5Y and SK-N-AS cells were cultured in RPMI 1640 media with 1 to 20muM concentration of arsenic trioxide. Apoptosis was measured with flow cytometry by staining with 7-aminoactinomycin D. Cell cycle was assessed by monitoring the DNA contents by flow cytometry. Arsenic trioxide induced cell morphologic changes were also observed with May-Grunwald-Giemsa stain under a light microscope.RESULTS:
Arsenic trioxide induced apoptosis in SH-SY5Y cells earlier in the same concentration and to a more severe degree with the same exposure time than in HL-60 cells. The apoptosis induced by arsenic trioxide was steeply increased to 79.3 10.1% at 24 hours and then maintained a plateau on 20muM concentration, while increasing steadily to 40.2 6.5% until 72 hours on 5muM concentration. The proliferating cell proportion in S/G2/M phase was decreased with arsenic trioxide concentration and with exposure time in both SH-SY5Y and HL-60 cells, especially more so with the SH-SY5Y cells. The cellularity was decreased and more apoptotic cells could be observed in the arsenic trioxide treatment group than in untreated control group.CONCLUSION:
As in acute promyelocytic leukemic cells, arsenic trioxide induced apoptosis and cell cycle arrest of proliferating phase in neuroblastoma cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Arsenic
/
DNA
/
Leukemia, Promyelocytic, Acute
/
Cell Cycle
/
Cell Line
/
Apoptosis
/
HL-60 Cells
/
Cell Cycle Checkpoints
/
Flow Cytometry
/
Neuroblastoma
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Pediatric Hematology-Oncology
Year:
2003
Type:
Article
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