A Proteomic Approach for Quantitative Analysis of Calcitonin Gene-related Peptides in the Cerebrospinal Fluid Obtained from a Rat Model of Chronic Neuropathic Pain / 대한통증학회지
The Korean Journal of Pain
;
: 112-118, 2008.
Article
in Korean
| WPRIM
| ID: wpr-115747
ABSTRACT
BACKGROUND:
This study was conducted to quantitatively analyze proteins associated with the calcitonin gene-related peptide (CGRP) in cerebrospinal fluid (CSF) that was obtained from a rat model of chronic neuropathic pain following administration of intrathecal CGRP(8-37).METHODS:
Male Sprague-Dawley rats (100-150 g, 5-6 wks) were divided into two groups, sham controls and neuropathic pain models. At the time of operation for neuropathic pain model, an intrathecal catheter was threaded through the intrathecal space. At 1 or 2 wks after the operation (maximum pain state), a test dose of 1, 5, 10, or 50 nM of CGRP(8-37) was injected into the intrathecal catheter and the CSF was then aspirated. Conventional proteomics to evaluate the CSF were then performed using high resolution 2-D, gel electrophoresis followed by computational image analysis and protein identification by mass spectrometry.RESULTS:
Treatment with CGRP(8-37) effectively alleviated mechanical allodynia in a dose dependent manner. The most effective response was obtained when a dose of 50 nM was administered, but significant differences were obtained following administration of only 5 nM CGRP(8-37). Furthermore, the results of the proteomic analysis were consistent with the experimental results. Specially we detected 30 differentially expressed spots in 7 images when 2-D gel electrophoresis was conducted. The intensity of 6 of these spots (spot number 20 and 26-30) was found decrease the CGRP(8-37) dose increased; therefore, these spots were evaluated by mass spectrometry. This analysis identified 2 different proteins, CGRP (spot numbers 26-30) and neurotensin-related peptide (spot number 20).CONCLUSIONS:
The results of this study suggest that CGRP plays a role in chronic central neuropathic pain and is a major target of chronic neuropathic pain management.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptides
/
Mass Spectrometry
/
Calcitonin
/
Electrophoresis, Gel, Two-Dimensional
/
Proteins
/
Calcitonin Gene-Related Peptide
/
Salicylamides
/
Rats, Sprague-Dawley
/
Proteomics
/
Electrophoresis
Limits:
Animals
/
Humans
/
Male
Language:
Korean
Journal:
The Korean Journal of Pain
Year:
2008
Type:
Article
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