Adhesion of CD40-stimulated Germinal Center B Cells to HK Cells Employs the CD11a/CD18-CD54 Interactions
Immune Network
;
: 176-181, 2003.
Article
in English
| WPRIM
| ID: wpr-116897
ABSTRACT
BACKGROUND:
The molecular basis of follicular dendritic cells (FDC)-germinal center (GC) B cell interaction is largely unknown, although this cellular interaction is thought to be important for the whole process of GC B cell differentiation.METHODS:
Using FDC-like cells, HK, and highly purified GC B cells, we attempted to identify the molecules that play critical roles in the interactions between FDC and B cells. GC B cells were co-cultured with HK cells and soluble CD154 in the presence or absence of various function-blocking monoclonal antibodies to examine their effect on GC B cell binding to HK cells and B cell proliferation.RESULTS:
Anti-CD11a and anti-CD54 antibodies inhibited GC B cell binding to HK cells while anti-CD49d and anti-CD106 antibodies did not. GC B cell proliferation was not impaired by the disruption of GC B cell-HK cell adherence.CONCLUSION:
Our results suggest that CD11a/CD18-CD54 interactions play an important roles in the initial binding of GC B cells to FDC and diffusible growth factors from FDC may be responsible the massive proliferation of GC B cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
B-Lymphocytes
/
Cell Communication
/
Cell Differentiation
/
Germinal Center
/
Dendritic Cells, Follicular
/
Intercellular Signaling Peptides and Proteins
/
Cell Proliferation
/
Antibodies
/
Antibodies, Monoclonal
Type of study:
Prognostic study
Language:
English
Journal:
Immune Network
Year:
2003
Type:
Article
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