Effect of Tumor Necrosis Factor-alpha Gene Transduced Bladder Tumor Cell Xenograft on Survival of C3H/He Mice / 대한비뇨기과학회지
Korean Journal of Urology
;
: 1333-1338, 1996.
Article
in Korean
| WPRIM
| ID: wpr-117016
ABSTRACT
OBJECTIVE:
To evaluate the effect of tumor necrosis factor-alpha (TNF-alpha)gene transduced bladder tumor cells on survival of C3H/He mice. MATERIALS ANDMETHODS:
The complete cDNA of TNF-alpha was introduced to mouse bladder tumor cell line(MBT-2) using a retroviral vector. Gene transfer and expression were confirmed by polymerase chain reaction(PCR) and reverse transcription polymerase chain reaction(RT-PCR). Produced TNF-alpha was measured by ELISA. TNF-alpha gene transduced and parental cells were xenografted in C3H/He mice. Implantation and growth rate and survival of experimental animals were analysed.RESULTS:
Successful gene transfer and expression were confirmed. TNF-G gene transduced MBT-2 cells secreted TNF-alpha at the concentration of 47.4pg/106cells/24hours. The implantation and growth rate of TNF-t gene transduced MBT-2 cells were much lower than those of parental cells and survival of experimental animals was different between TNF-t gene transduced MBT-2 and parental cells(P<0.05).CONCLUSION:
This study shows potential advantages of localized TNF-a secretion to induce potent antitumorigenic response. Tumor cell targeted TNF-a gene therapy could become a useful tool in treatment of bladder cancer.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Parents
/
Urinary Bladder
/
Urinary Bladder Neoplasms
/
Enzyme-Linked Immunosorbent Assay
/
Genetic Therapy
/
Zidovudine
/
Tumor Necrosis Factor-alpha
/
DNA, Complementary
/
Reverse Transcription
/
Heterografts
Limits:
Animals
/
Humans
Language:
Korean
Journal:
Korean Journal of Urology
Year:
1996
Type:
Article
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