Folate Receptor Targeted Imaging Using Poly (ethylene glycol)-folate: In Vitro and In Vivo Studies
Journal of Korean Medical Science
;
: 405-411, 2007.
Article
in English
| WPRIM
| ID: wpr-118036
ABSTRACT
The aim of this study was to ascertain the folate receptor (FR) targetability by an in vitro study and to acquire FR-targeted images in vivo models by using synthetic folate conjugates. PEG-folate was synthesized and labeled with (99m)Tc and fluorescein isothiocynate (FITC). Cell uptake studies were carried out in KB cells (FR-positive) and A549 cells (FR-negative) using FITC- and the (99m)Tc-labeled conjugates. The radiolabeled conjugate was intravenously injected to KB tumor xenografted mice. After it was injected, gamma images were recorded at 30 min, 1, 2, 3 and 4 hr. Cell uptake studies showed a difference between the KB cells and the A549 cells by flow cytometry analysis and gamma counting. On in vivo images, the tumor-tonormal muscle ratio was greater than 4. It ascertained that the PEG-folate conjugate specifically binds to the FR expressed on tumor cells in vitro. Moreover, it was possible to acquire the FR-targeted gamma images using PEG-folate conjugates in tumor models.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polyethylene Glycols
/
Time Factors
/
Image Processing, Computer-Assisted
/
Carrier Proteins
/
Technetium
/
Fluorescein-5-isothiocyanate
/
Receptors, Cell Surface
/
Cell Line, Tumor
/
Folic Acid
/
Mice, Nude
Type of study:
Prognostic study
Limits:
Animals
/
Female
/
Humans
Language:
English
Journal:
Journal of Korean Medical Science
Year:
2007
Type:
Article
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