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Reduction of the HIV Seroconversion Window Period and False Positive Rate by Using ADVIA Centaur HIV Antigen/Antibody Combo Assay
Annals of Laboratory Medicine ; : 420-425, 2013.
Article in English | WPRIM | ID: wpr-118366
ABSTRACT

BACKGROUND:

Early diagnosis of HIV infection reduces morbidity and mortality. Fourth-generation HIV detection assays are more sensitive because they can detect p24 antigen as well as anti-HIV antibodies. In this study, we evaluated the performance of a new fourth-generation ADVIA Centaur HIV antigen/antibody combo (CHIV) assay (Siemens Healthcare Diagnostics Inc., USA) for early detection of HIV infection and reduction of false positive rate.

METHODS:

Four seroconversion panels were included. The third-generation ADVIA Centaur HIV 1/O/2 enhanced (EHIV) assay (Siemens Healthcare Diagnostics Inc., USA) and fourth-generation CHIV assay were used to test each panel for HIV infection. The presence of antigen was confirmed using HIV p24 antigen assay. To evaluate false-positivity and specificity, 54 HIV false-positive and HIV-negative serum samples from 100 hospitalized patients and 600 healthy subjects were included.

RESULTS:

Compared to the EHIV assay, the CHIV assay had a shorter window for three of the seroconversion panels a difference of 10 days and two bleeds in one panel, and 4 days and one bleed in the other two panels. Only 34 of the 54 (63%) samples known to yield false-positive results by EHIV assay had repeatedly yielded reactive results in the CHIV assay. One of the 600 healthy subjects had a false-positive result with the CHIV assay; thus, the specificity was 99.85% (699/700). CHIV accurately determined the reactive results for the HIV-confirmed serum samples from known HIV patients and Korea Food & Drug Administration (KFDA) panels.

CONCLUSIONS:

The new fourth-generation ADVIA Centaur HIV assay is a sensitive and specific assay that shortens the serological window period and allows early diagnosis of HIV infection.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Reagent Kits, Diagnostic / Time Factors / HIV Antibodies / Sensitivity and Specificity / HIV Seropositivity / HIV Core Protein p24 / False Positive Reactions / Republic of Korea Type of study: Diagnostic study / Screening study Limits: Female / Humans / Male / Pregnancy Country/Region as subject: Asia Language: English Journal: Annals of Laboratory Medicine Year: 2013 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Reagent Kits, Diagnostic / Time Factors / HIV Antibodies / Sensitivity and Specificity / HIV Seropositivity / HIV Core Protein p24 / False Positive Reactions / Republic of Korea Type of study: Diagnostic study / Screening study Limits: Female / Humans / Male / Pregnancy Country/Region as subject: Asia Language: English Journal: Annals of Laboratory Medicine Year: 2013 Type: Article