Identification of HLA-B*5001 in Korean Population / 대한소아혈액종양학회지
Korean Journal of Pediatric Hematology-Oncology
;
: 323-330, 2001.
Article
in Korean
| WPRIM
| ID: wpr-118583
ABSTRACT
PURPOSE:
HLA (human leukocyte antigen)-class I genes are highly polymorphic, play many roles in organ and bone marrow transplantation. HLA-B is the most polymorphic class I locus with 414 alleles. HLA-class I typing, which is based on serologic method, has been used until recently. The development of molecular biological techniques make it possible to define the genotypes of HLA genes.METHODS:
Analyses of HLA-B genotyping on 1,000 UCB (Umbilical Cord Blood) samples which were considered to be HLA-B homozygote or blank were performed by ARMS-PCR (Amplification Refractory Mutation System-PCR) method and direct sequencing.RESULTS:
We could identify HLA-B*5001 which was known to be absent in Koreans.CONCLUSION:
It is strongly suggested that HLA-B homozygote should be confirmed to the DNA level especially in cases of donor selection for the unrelated bone marrow transplantation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA
/
HLA-B Antigens
/
Bone Marrow Transplantation
/
Donor Selection
/
Alleles
/
Fetal Blood
/
Genotype
/
Homozygote
/
Leukocytes
Type of study:
Diagnostic study
Language:
Korean
Journal:
Korean Journal of Pediatric Hematology-Oncology
Year:
2001
Type:
Article
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