Saccharomyces boulardii Activates Expression of Peroxisome Proliferator-activated Receptor-gamma in HT-29 Cells / 대한소화기학회지
The Korean Journal of Gastroenterology
;
: 328-334, 2005.
Article
in Korean
| WPRIM
| ID: wpr-118713
ABSTRACT
BACKGROUND/AIMS:
Saccharomyces boulardii (S. boulardii) has been reported to be beneficial in the treatment of inflammatory bowel disease, however, little is known about its mechanism of action. Peroxisome proliferator- activated receptor-gamma (PPAR-gamma) is recently found to regulate inflammation in intestinal epithelial cells. We hypothesized that the anti-inflammatory effects of S. boulardii are mediated, in part, through PPAR-gamma. To test this hypothesis, we examined the ability of S. boulardii to modulate the expression of PPAR-gamma in human colon cells.METHODS:
Effects of S. boulardii on survival and proliferation of HT-29 human colon cells were assessed by MTT and [3H]thymidine incorporation assays. PPAR-gamma expression was assessed by Western blot and RT-PCR. Induction of interleukin-8 (IL-8) expression by tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), or lipopolysaccharide (LPS) was assessed by RT-PCR.RESULTS:
S. boulardii did not affect viability and proliferation of HT-29 cells. S. boulardii up-regulated PPAR-gamma expression at both mRNA and protein levels. Pretreatment of HT-29 cells with S. boulardii blocked PPAR-gamma down-regulation by TNF-alpha, IL-1beta, or LPS, whereas it ameliorated IL-8 response to these proinflammatory factors.CONCLUSIONS:
S. boulardii stimulates PPAR-gamma expression and reduces response of human colon cells to proinflammatory cytokines.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Saccharomyces
/
Gene Expression
/
Lipopolysaccharides
/
Interleukin-8
/
Interleukin-1
/
Colon
/
HT29 Cells
/
PPAR gamma
/
Cell Proliferation
Limits:
Humans
Language:
Korean
Journal:
The Korean Journal of Gastroenterology
Year:
2005
Type:
Article
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