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Rapid Detection and Identification of Wuchereria bancrofti, Brugia malayi, B. pahangi, and Dirofilaria immitis in Mosquito Vectors and Blood Samples by High Resolution Melting Real-Time PCR
The Korean Journal of Parasitology ; : 645-650, 2013.
Article in English | WPRIM | ID: wpr-118761
ABSTRACT
A simple, rapid, and high-throughput method for detection and identification of Wuchereria bancrofti, Brugia malayi, Brugia pahangi, and Dirofilaria immitis in mosquito vectors and blood samples was developed using a real-time PCR combined with high-resolution melting (HRM) analysis. Amplicons of the 4 filarial species were generated from 5S rRNA and spliced leader sequences by the real-time PCR and their melting temperatures were determined by the HRM method. Melting of amplicons from W. bancrofti, B. malayi, D. immitis, and B. pahangi peaked at 81.5+/-0.2degrees C, 79.0+/-0.3degrees C, 76.8+/-0.1degrees C, and 79.9+/-0.1degrees C, respectively. This assay is relatively cheap since it does not require synthesis of hybridization probes. Its sensitivity and specificity were 100%. It is a rapid and technically simple approach, and an important tool for population surveys as well as molecular xenomonitoring of parasites in vectors.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Parasitology / Wuchereria bancrofti / Blood / Brugia / RNA, Ribosomal, 5S / Sensitivity and Specificity / RNA, Helminth / Transition Temperature / Dirofilaria immitis / Real-Time Polymerase Chain Reaction Type of study: Diagnostic study Limits: Animals / Humans / Male Language: English Journal: The Korean Journal of Parasitology Year: 2013 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Parasitology / Wuchereria bancrofti / Blood / Brugia / RNA, Ribosomal, 5S / Sensitivity and Specificity / RNA, Helminth / Transition Temperature / Dirofilaria immitis / Real-Time Polymerase Chain Reaction Type of study: Diagnostic study Limits: Animals / Humans / Male Language: English Journal: The Korean Journal of Parasitology Year: 2013 Type: Article