Rapid Detection and Identification of Wuchereria bancrofti, Brugia malayi, B. pahangi, and Dirofilaria immitis in Mosquito Vectors and Blood Samples by High Resolution Melting Real-Time PCR
The Korean Journal of Parasitology
;
: 645-650, 2013.
Article
in English
| WPRIM
| ID: wpr-118761
ABSTRACT
A simple, rapid, and high-throughput method for detection and identification of Wuchereria bancrofti, Brugia malayi, Brugia pahangi, and Dirofilaria immitis in mosquito vectors and blood samples was developed using a real-time PCR combined with high-resolution melting (HRM) analysis. Amplicons of the 4 filarial species were generated from 5S rRNA and spliced leader sequences by the real-time PCR and their melting temperatures were determined by the HRM method. Melting of amplicons from W. bancrofti, B. malayi, D. immitis, and B. pahangi peaked at 81.5+/-0.2degrees C, 79.0+/-0.3degrees C, 76.8+/-0.1degrees C, and 79.9+/-0.1degrees C, respectively. This assay is relatively cheap since it does not require synthesis of hybridization probes. Its sensitivity and specificity were 100%. It is a rapid and technically simple approach, and an important tool for population surveys as well as molecular xenomonitoring of parasites in vectors.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Parasitology
/
Wuchereria bancrofti
/
Blood
/
Brugia
/
RNA, Ribosomal, 5S
/
Sensitivity and Specificity
/
RNA, Helminth
/
Transition Temperature
/
Dirofilaria immitis
/
Real-Time Polymerase Chain Reaction
Type of study:
Diagnostic study
Limits:
Animals
/
Humans
/
Male
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2013
Type:
Article
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