Construction of MAGE - 3 Expressing Plasmid for Development of DNA Vaccine Encoding MAGE - 3 Cancer Antigen / 대한암학회지
Journal of the Korean Cancer Association
;
: 191-199, 2000.
Article
in Korean
| WPRIM
| ID: wpr-11903
ABSTRACT
PURPOSE:
The spectrum of melanoma antigen gene (MAGE)-expressing tumor is very wide and the gene of MAGE express antigens that are targets for specific recognition by cytotoxic T lymphocytes derived from tumor-bearing patients. All of these characteristics represent MAGE as tumor vaccine can be useful for cancer prevention or treatment. Here, we detected MAGE-3 gene expression in cancer cell lines and evaluated recombinant MAGE-3 protein producibility of MAGE plasmid to develope MAGE DNA vaccine. MATERIALS ANDMETHODS:
MAGE-3 gene expression of cancer cell lines was evaluated by reverse transcription-polymerase chanin reaction (RT-PCR). Two kinds of MAGE-3 expressing plasmids were constructed and their MAGE-3 protein producibility was evaluated by immunohistochemistry and immunoblotting using monoclonal anti-MAGE-3 antibody.RESULTS:
Among 13 cell lines, SNU484, AMC-HN-3, AMC-HN-4, AMC-HN-7, HeLa, NCI H1703 and HT29 expressed MAGE-3 mRNA. In order to make MAGE plasmid, cDNA that showed 100% DNA homology with MAGE-3 gene was cloned into pcDNA 3 plasmid and pSecTag plasmid. Intracytoplasmic and secretory recombinant MAGE-3 was produced by MAGE-3 containing pcDNA 3 plasmid and pSecTag plasmid, respectively.CONCLUSION:
In this study, we showed high expression frequency of MAGE-3 in cancer cell line, and established two kinds of plasmid that produce recombinant MAGE-3 in cell lines. We expect these plasmids will be used in cancer treatment or MAGE-3 function study in future.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
DNA
/
RNA, Messenger
/
Immunohistochemistry
/
T-Lymphocytes, Cytotoxic
/
Immunoblotting
/
Gene Expression
/
Cell Line
/
Clone Cells
/
DNA, Complementary
Limits:
Humans
Language:
Korean
Journal:
Journal of the Korean Cancer Association
Year:
2000
Type:
Article
Similar
MEDLINE
...
LILACS
LIS