Usefulness of the IMI Channel of Sysmex SE-9000(TM) Automated Hematology Analyzer Predicting the Optimal Timing of Peripheral Blood Stem Cell Harvest / 임상검사와정도관리
Journal of Laboratory Medicine and Quality Assurance
;
: 223-230, 2003.
Article
in Korean
| WPRIM
| ID: wpr-119815
ABSTRACT
BACKGROUND:
An essential prerequisite for successful procurement of sufficient peripheral blood stem cells (PBSC) for engraftment is the optimal timing of collection. The Sysmex SE-9000 automated hematology analyzer provides the immature information (IMI) channel for the identification and counting PBSC. In this study, The optimal timing of PBSC collection was studied using IMI channel.METHODS:
193 peripheral blood stem cell collections were performed from 52 patients with hematologic disorders or solid tumors and 15 donors. Pre-harvest peripheral blood WBC, mononuclear cells (MNC) and IMI were tested and compared with CD34+ cell count and CFU-GM count of harvested products.RESULTS:
Peripheral blood WBC and MNC count showed a weak correlation with CD34+ cell yield (r=0.38, P1x10(6)/kg with sensitivity of 88.7%. Positive and negative predictive values of IMI >465/microliter for CD34+ cell >1x10(6)/kg were 65.5% and 87.5%, respectively.CONCLUSIONS:
The automated IMI might be used as a simple and efficient indicator of PBSC mobilization and applying variable cutoff values of IMI would be a useful tool to predict the optimal timing of PBSC collection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Stem Cells
/
Tissue Donors
/
Cell Count
/
ROC Curve
/
Granulocyte-Macrophage Progenitor Cells
/
Hematology
Type of study:
Prognostic study
Limits:
Humans
Language:
Korean
Journal:
Journal of Laboratory Medicine and Quality Assurance
Year:
2003
Type:
Article
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