Obesity aggravates the joint inflammation in a collagen-induced arthritis model through deviation to Th17 differentiation
Experimental & Molecular Medicine
;
: 424-431, 2012.
Article
in English
| WPRIM
| ID: wpr-119839
ABSTRACT
White fat cells secrete adipokines that induce inflammation and obesity has been reported to be characterized by high serum levels of inflammatory cytokines such as IL-6 and TNF-alpha. Rheumatoid arthritis (RA) is a prototype of inflammatory arthritis, but the relationship between RA and obesity is controversial. We made an obese inflammatory arthritis model obese collagen-induced arthritis (CIA). C57BL/6 mice were fed a 60-kcal high fat diet (HFD) from the age of 4 weeks and they were immunized twice with type II collagen (CII). After immunization, the obese CIA mice showed higher arthritis index scores and histology scores and a more increased incidence of developing arthritis than did the lean CIA mice. After treatment with CII, mixed lymphocyte reaction also showed CII-specific response more intensely in the obese CIA mice than lean CIA. The anti-CII IgG and anti-CII IgG2a levels in the sera of the obese CIA mice were higher than those of the lean CIA mice. The number of Th17 cells was higher and the IL-17 mRNA expression of the splenocytes in the obese CIA mice was higher than that of the lean CIA mice. Obese CIA mice also showed high IL-17 expression on synovium in immunohistochemistry. Although obesity may not play a pathogenic role in initiating arthritis, it could play an important role in amplifying the inflammation of arthritis through the Th1/Th17 response. The obese CIA murine model will be an important tool when we investigate the effect of several therapeutic target molecules to treat RA.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Arthritis, Experimental
/
Gene Expression
/
Cell Differentiation
/
Interleukin-6
/
Tumor Necrosis Factor-alpha
/
Interleukin-17
/
Collagen Type II
/
Disease Models, Animal
/
Adipokines
/
Th17 Cells
Type of study:
Prognostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2012
Type:
Article
Similar
MEDLINE
...
LILACS
LIS