Involvement of Nuclear Factor Kappa B in High-Fat Diet-Related Pancreatic Fibrosis in Rats
Gut and Liver
;
: 381-387, 2012.
Article
in English
| WPRIM
| ID: wpr-119845
ABSTRACT
BACKGROUND/AIMS:
High-fat diets contribute to pancreatic fibrogenesis, but the pathogenesis remains unclear. This study investigated the role of nuclear factor kappa B (NF-kappaB) in high-fat diet-induced pancreatic fibrosis in rats.METHODS:
Male Wistar rats were fed a high-fat diet or standard normal chow for 20 weeks. Pancreatic fibrosis was determined by Sirius red staining. Immunohistochemical staining, reverse transcription-polymerase chain reaction and Western blotting were used to identify NF-kappaB-associated genes or protein expressions.RESULTS:
Inflammation, fat deposition, pancreatic stellate cell activation and fibrosis were observed in the pancreases of the high-fat diet group. NF-kappaB subunit p65 (NF-kappaB/p65) expression was localized to the nucleus, and intercellular adhesion molecule 1 (ICAM-1) was over-expressed. Pancreatic gene expression levels of NF-kappaB/p65, ICAM-1 and tumor necrosis factor alpha were all elevated significantly in rats fed a high-fat diet compared with control rats. Western blotting also revealed significantly increased levels of ICAM-1 and nuclear NF-kappaB/p65 in rats fed high-fat diets comparison with control rats.CONCLUSIONS:
NF-kappaB is involved in high-fat diet-related pancreatic fibrosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pancreas
/
Fibrosis
/
Gene Expression
/
Blotting, Western
/
NF-kappa B
/
Tumor Necrosis Factor-alpha
/
Rats, Wistar
/
Intercellular Adhesion Molecule-1
/
Pancreatic Stellate Cells
/
Diet, High-Fat
Type of study:
Prognostic study
Limits:
Animals
/
Humans
/
Male
Language:
English
Journal:
Gut and Liver
Year:
2012
Type:
Article
Similar
MEDLINE
...
LILACS
LIS