Comparison of Intra-gall Bladder and Intra-common Bile Duct Injection of Collagenase Solution for Mouse Pancreatic Islet Isolation
Journal of the Korean Surgical Society
;
: 8-12, 2007.
Article
in Korean
| WPRIM
| ID: wpr-120087
ABSTRACT
PURPOSE:
A high yield of viable pancreatic islets is an essential prerequisite for the study of pancreatic islet transplantation. The purpose of this study is to compare the yield between intra-gall bladder (intra-GB) and intra-common bile duct (intra-CBD) injection of collagenase solution for isolation of mouse pancreatic islets.METHODS:
The mice were divided into two groups, the intra-GB and intra-CBD groups, and each group included twelve mice, respectively. Collagenase solution was injected via the gallbladder in the intra-GB group mice, while this was done via the common bile duct in the intra-CBD group. After removal and digestion of the mouse pancreases, the pancreatic islets were isolated by Ficoll density gradient centrifugation and hand picking.RESULTS:
The intra-GB group yielded 121.67+/-39.86 IEQs, and the intra-CBD group reveled 168.17+/-29.23 IEQs. There was a statistically significant difference in islet yield between the two groups (P=0.005, Mann-Whitney Test). The purities of the isolated islets were 86.42+/-3.99% for the intra-GB group and 87.17+/-4.47% for the intra-CBD group, and there was no difference between the two groups (P=0.755, Mann- Whitney Test).CONCLUSION:
Both the intra-GB and intra-CBD groups yielded an average of >120 IEQs. However, the intra-CBD group revealed a higher yield than the intra-GB group for isolating mouse pancreatic islets.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pancreas
/
Bile
/
Bile Ducts
/
Urinary Bladder
/
Centrifugation, Density Gradient
/
Islets of Langerhans
/
Collagenases
/
Common Bile Duct
/
Digestion
/
Ficoll
Limits:
Animals
Language:
Korean
Journal:
Journal of the Korean Surgical Society
Year:
2007
Type:
Article
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