Isolation and Characterization of Human Muscle Cells / 대한류마티스학회지
The Journal of the Korean Rheumatism Association
;
: 64-69, 1996.
Article
in Korean
| WPRIM
| ID: wpr-121193
ABSTRACT
OBJECTIVES:
To isolate and culture of human muscle cells by manipulating culture conditions.METHODS:
Muscle samples were obtained during total hip replacement or bedside muscle biopsy. We isolated myoblasts from freshly obtained human muscle tissue by trypsin and collagenase digesion.RESULTS:
Selective isolation of myoblasts was identified through histologic examination by light-microscope. We identified monoc]onal antibodies(Leu-19) as molecular markers in human skeletal muscle were expressed.CONCLUSIONS:
This study demonstrated that it had been possible to culture morphologically and immunelogically identifiable myoblasts isolated from human skeletal muscle tissue.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Biopsy
/
Trypsin
/
Collagenases
/
Muscle, Skeletal
/
Arthroplasty, Replacement, Hip
/
Muscle Cells
/
Myoblasts
Limits:
Humans
Language:
Korean
Journal:
The Journal of the Korean Rheumatism Association
Year:
1996
Type:
Article
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