Effect of Three Dimensional Culture Using Alginate-Fibrin Beads on the Chondrogenic Differentiation of Rabbit's Perichondrial Cells / 대한정형외과연구학회지
Journal of Korean Orthopaedic Research Society
;
: 1-6, 2003.
Article
in Korean
| WPRIM
| ID: wpr-121355
ABSTRACT
PURPOSE:
To evaluate the usefulness of three dimensional culture using alginate-fibrin beads on the chondrogenic differentiation of rabbit's perichondrial cells. MATERIALS ANDMETHODS:
Rib perichondrial cells from rabbit expanded by monolayer culture were cultured in monolayer, in alginate bead, and in alginate-fibrin beads. Reverse transcription polymerase chain reaction (RT-PCR) for type I, II, X collagen, and aggrecan was performed at 3 weeks after culture. At that time, we removed the alginate component from the alginate-fibrin bead. Then, the cell-fibrin beads were transplanted into the partial physeal defect of proximal tibia, and some beads were cultured on for additional 3 weeks for RT-PCR. Histologic examination was performed at 2, 4, 8, 12, and 16 weeks after operation.RESULTS:
At 3 weeks, type II collagen gene expression was maintained regardless of the culture system used. However, at 6 weeks it was maintained only in three-dimensional culture system using alginate beads and fibrin beads. Histologic examination showed that the implanted perichondrial cell-fibrin beads formed small nest composed of chondrocyte-like cells and matrix.CONCLUSION:
These results suggest that alginate-fibrin beads may be used as a biodegradable scaffold for cartilage engineering using perichondrial cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ribs
/
Tibia
/
Fibrin
/
Cartilage
/
Gene Expression
/
Polymerase Chain Reaction
/
Collagen
/
Collagen Type II
/
Reverse Transcription
/
Aggrecans
Language:
Korean
Journal:
Journal of Korean Orthopaedic Research Society
Year:
2003
Type:
Article
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