Expression and localization of Rdd proteins in Xenopus embryo / 대한해부학회지
Anatomy & Cell Biology
;
: 18-27, 2014.
Article
in English
| WPRIM
| ID: wpr-121388
ABSTRACT
The previous study has shown that repeated D domain-like (Rdd) proteins, a group of novel secretory proteins consisting of repeated domains of a cysteine-rich sequence, are involved in the process of blood vessel formation in Xenopus embryo. We performed further experiments to examine the localization of Rdd proteins in embryogenesis. Detection of tagged Rdd proteins expressed in blastomeres showed that Rdd proteins formed a high molecular weight complex and existed in the extracellular space. A rabbit antibody against the Rdd synthetic peptide identified a single band of 28 kD in embryonic tissue extract. By whole-mount immunostaining analysis, signal was detected in the regions of inter-somites, vitelline veins, and branchial arches at the tailbud stage. Staining of Rdd was remarkably reduced in the embryos injected with vascular endothelial growth factor Morpholino. We suggest that Rdd proteins interact with a molecule(s) associated with vascular precursor cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Veins
/
Xenopus
/
Blastomeres
/
Blood Vessels
/
Branchial Region
/
Vascular Endothelial Growth Factor A
/
Vitellins
/
Embryonic Development
/
Embryonic Structures
/
Extracellular Space
Type of study:
Prognostic study
Limits:
Pregnancy
Language:
English
Journal:
Anatomy & Cell Biology
Year:
2014
Type:
Article
Similar
MEDLINE
...
LILACS
LIS