Analysis of Amelogenin Gene & Short Tandem Repeat(STR) Locus F13A01, LPL from Pulpless Teeth Dentin / 대한법의학회지

ABSTRACT

Theoretically, if the odontoblastic layer on the pulpal surface of the dentin is removed, it is impossible to find nucleus after the teeth has undergone endodontic therapy. So dentin does not contain genomic DNA. But it is in existence the possibility that blood permeates into the dentin by endodontic therapy and trauma. Therefore DNA in blood can be detected from the dentin layer. The purpose of this investigation is to evaluate the possibility of individual identification after detection of DNA in the dentin of endodontic created teeth. The authors isolated the human DNA from 40 endodontic treated teeth, performed AmpFLPs by PCR and electrophoresed for detection of STR loci F13A01, LPL gene and X-Y homologous amelogenin gene. The following results were obtained 1) DNA extraction was possible in 19 endodontic treated teeth out of 40. 2) Sex determination was possible in 12 endodontic treated teeth out of 40 by detection of X-Y homologous amelogenin gene 3) F13A01 locus was detected in 6 endodontic treated teeth out of 40, observed 4 alleles(3.2, 4, 5, 6) and 5 genotypes(3.2-3.2, 3.2-4, 3.2-5, 4-5, 6-6). 4) LPL locus was detected in 7 endodontic treated teeth out of 40, observed 3 alleles(10, 11, 12) and 3 genotypes(10-10, 10-12, 11-12). From the above results DNA extraction, sex determination, amplification of STR locus F13A01, and LPL gene were possible in the endodontic treated teeth and it was suggested that endodontic treated teeth were useful and applicatable as molecular biological samples for individual identification.