The Modulation of IL-12 by Phosphodiesterase IV Inhibitor and Subsequent Changes of Th-1, Th-2 Cytokines in Human Endometrial Tissue / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 1500-1509, 2003.
Article
in Korean
| WPRIM
| ID: wpr-121637
ABSTRACT
OBJECTIVE:
To assess the capability of phosphodiesterase type IV inhibitor (rolipram) to suppress IL-12 in human decidua and the subsequent changes of Th-2 cytokine (IL-10) and Th-1 cytokine (TNF-alpha).METHODS:
Decidual tissues of 10 first-trimester pregnant women and 10 first-trimester pregnant women diagnosed as missed abortion were collected by dilatation and currettage. The decidual tissues were treated with rolipram for 6 hours. Protein and mRNA expression in the tissues were analysed by western blotting, immunohistochemistry and reverse transcription-polymerase chain reaction.RESULTS:
Rolipram, in the concentration above 1 microgram/ml, could decrease the expression of IL-12p35 (control 46.37+/-7.38, rolipram 24.34+/-8.46) and IL-12p40 mRNA (control 31.7+/-5.8, rolipram 14.9+/-4.6) and protein (control 52.4+/-8.9, rolipram 40.9+/-12.1). However, the expression of IL-10 and TNF-alpha mRNA and protein did not changed by rolipram. There was no difference in the cytokine expression pattern between the decidual tissues of normal pregnancy and missed abortion.CONCLUSION:
Rolipram, the phosphodiesterase type IV inhibitor, could induce the decrease of IL-12 in human decidua. In human decidual tissue, unlike other human tissues, the decrease of IL-12 by rolipram did not modulate other Th-1/Th-2 cytokines. Inability of IL-12 to modulate other Th-1/Th-2 cytokines might be related with unique cytokine network in human decidua rather than its small extent of decrease.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Immunohistochemistry
/
Blotting, Western
/
Cytokines
/
Tumor Necrosis Factor-alpha
/
Interleukin-10
/
Abortion, Missed
/
Interleukin-12
/
Rolipram
/
Decidua
Limits:
Female
/
Humans
/
Pregnancy
Language:
Korean
Journal:
Korean Journal of Obstetrics and Gynecology
Year:
2003
Type:
Article
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