Developmental Differences in NMDA Neurototoxicity and the Expression of NMDA Receptor 2B Subunit of Rat Hippocampus
Journal of the Korean Neurological Association
;
: 364-371, 2007.
Article
in Korean
| WPRIM
| ID: wpr-122095
ABSTRACT
BACKGROUND:
The developing brain has a distinctive set of characteristics that make it have different susceptibility to excitotoxins. Using primary tissue cultures of rat hippocampus, we investigated the developmental susceptibility to N-methyl-D-aspartic acid (NMDA)-induced cell death at various days in vitro in relation to the appearance of Bcl-2 protein and NMDA receptor 2B subunit.METHODS:
Six, 12, and 18 days-in-vitro (DIV) hippocampal tissue cultures derived from 7-day-old Sprague-Dawley rat pups were used. Each group was treated with 100 micrometer NMDA in 5% CO2 incubator at 36 degrees C for 30 min. A western blot was then performed for the NeuN, Bcl-2 and NMDA receptor 2B subunit and propidium Iodide (PI) staining.RESULTS:
The NeuN and Bcl-2 were most highly expressed in 12 DIV tissues. The reductions of the NeuN and Bcl-2 protein expressions by NMDA were significant at the 12 and the 18 DIV tissues, but less at 6 DIV tissues (p<0.05). The PI staining showed that the area of fluorescence of the 7 DIV tissues after NMDA exposure was less than the DIV 13 and 19 tissues. Without NMDA treatment, the NMDA receptor 2B subunit protein expressions at the 6 DIV tissues were highest and decreased with maturation.CONCLUSIONS:
These results suggest that the immature tissues were more resistant to NMDA toxicity than the mature tissues, and further studies are needed to establish its relationship with the Bcl-2 protein and NMDA receptor 2B subunit.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Propidium
/
Brain
/
Blotting, Western
/
N-Methylaspartate
/
Cell Death
/
Rats, Sprague-Dawley
/
Fluorescence
/
Hippocampus
/
Incubators
/
Neurotoxins
Limits:
Animals
Language:
Korean
Journal:
Journal of the Korean Neurological Association
Year:
2007
Type:
Article
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