Enhanced skin wound healing by a sustained release of growth factors contained in platelet-rich plasma
Experimental & Molecular Medicine
;
: 622-629, 2011.
Article
in English
| WPRIM
| ID: wpr-122147
ABSTRACT
Platelet-rich plasma (PRP) contains growth factors that promote tissue regeneration. Previously, we showed that heparin-conjugated fibrin (HCF) exerts the sustained release of growth factors with affinity for heparin. Here, we hypothesize that treatment of skin wound with a mixture of PRP and HCF exerts sustained release of several growth factors contained in PRP and promotes skin wound healing. The release of fibroblast growth factor 2, platelet-derived growth factor-BB, and vascular endothelial growth factor contained in PRP from HCF was sustained for a longer period than those from PRP, calcium-activated PRP (C-PRP), or a mixture of fibrin and PRP (F-PRP). Treatment of full-thickness skin wounds in mice with HCF-PRP resulted in much faster wound closure as well as dermal and epidermal regeneration at day 12 compared to treatment with either C-PRP or F-PRP. Enhanced skin regeneration observed in HCF-PRP group may have been at least partially due to enhanced angiogenesis in the wound beds. Therefore, this method could be useful for skin wound treatment.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Regeneration
/
Skin
/
Wound Healing
/
RNA, Messenger
/
Fibrin
/
Heparin
/
Blotting, Western
/
Fibroblast Growth Factor 2
/
Immunoenzyme Techniques
/
Dermis
Limits:
Animals
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2011
Type:
Article
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