Your browser doesn't support javascript.
loading
Neuroprotective Effect of Cycloheximide on Hypoxic-Ischemic Brain Injury in Neonatal Rats
Journal of Korean Medical Science ; : 337-341, 2006.
Article in English | WPRIM | ID: wpr-12252
ABSTRACT
This study was done to determine the neuroprotective effect of cycloheximide on neonatal hypoxic-ischemic brain injury. Seven day-old newborn rat pups were subjected to 90 min of 8% oxygen following a unilateral carotid artery ligation. The extent of cerebral infarction was evaluated at 1 and 4 week of recovery. Apoptosis was identified by performing terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining and flow cytometry with a combination of fluoresceinated annexin V and propidium iodide. Brain infarction area was significantly increased at 4 week compared to 1 week after hypoxia-ischemia in the control group. With cycloheximide treatment, the number of TUNEL positive cells in the ipsilateral cerebral cortex at 48 hr and peri-infarct area at 1 and 4 week of recovery was significantly reduced, both apoptotic and necrotic cells by flow cytometry 48 hr after the injury were significantly reduced, and the extent of cerebral infarction at 1 and 4 week of recovery was also significantly attenuated compared to the hypoxia-ischemia control group. In summary, our data suggest that apoptosis plays an important role in the development of delayed infarction, and inhibition of apoptosis with cycloheximide significantly reduces the ensuing cerebral infarction in a newborn rat pup model of cerebral hypoxia-ischemia.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Propidium / Time Factors / Rats, Sprague-Dawley / Apoptosis / Annexin A5 / Neuroprotective Agents / In Situ Nick-End Labeling / Brain Infarction / Hypoxia-Ischemia, Brain / Cycloheximide Type of study: Prognostic study Limits: Animals Language: English Journal: Journal of Korean Medical Science Year: 2006 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Propidium / Time Factors / Rats, Sprague-Dawley / Apoptosis / Annexin A5 / Neuroprotective Agents / In Situ Nick-End Labeling / Brain Infarction / Hypoxia-Ischemia, Brain / Cycloheximide Type of study: Prognostic study Limits: Animals Language: English Journal: Journal of Korean Medical Science Year: 2006 Type: Article