Effect of Rho Kinase Inhibitor on the Production of Nitric Oxide in Trabecular Meshwork Cells
Journal of the Korean Ophthalmological Society
;
: 650-656, 2016.
Article
in Korean
| WPRIM
| ID: wpr-122532
ABSTRACT
PURPOSE:
To investigate the effects of Rho kinase (ROCK) inhibitor on the production of nitric oxide (NO) and expression of endothelial nitric oxide synthase (eNOS) in cultured human trabecular meshwork cells (HTMC).METHODS:
Primarily cultured HTMC were exposed to 0 µM, 10 µM or 100 µM Y-27632 for 3 days and NO production was assessed using Griess assay. After 24 hours, the effect of Y-27632 on the contraction of collagen matrix and the permeability of the HTMC monolayer was determined. The expression of eNOS mRNA was assessed using reverse transcription-polymerase chain reaction (RT-PCR) and cellular survival with the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay.RESULTS:
In HTMC, 10 µM and 100 µM Y-27632 significantly increased NO production after 1 day and 3 days (p = 0.020 and 0.001, respectively). At 1 day after exposure, Y-276320 significantly relaxed the collagen matrix and increased the permeability of the HTMC monolayer (all p = 0.001) and the eNOS mRNA expression (p = 0.039).CONCLUSIONS:
Increased NO production may play a role in the mechanism of increased trabecular outflow associated with ROCK inhibitor.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Permeability
/
Trabecular Meshwork
/
RNA, Messenger
/
Collagen
/
Nitric Oxide Synthase Type III
/
Rho-Associated Kinases
/
Nitric Oxide
Limits:
Humans
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2016
Type:
Article
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