Effect of Autologous Peripheral Blood Mononuclear Cell Transplantation on Patients with Chronic Ischemic Limb

ABSTRACT

PURPOSE: In the present study, the effects of autologous peripheral blood mononuclear cells (PBMNC) transplantation after granulocyte colony stimulation factor (G-CSF)-induced mobilization was investigated in patient with severe peripheral arterial disease. METHOD: 4 cases were enrolled 3 cases are atherosclerosis obliterance and 1 case is thromboangitis obliterance. Following administration of G-CSF (10microgram/kg, maximum 600microgram), 4 times for 4 days. PBMNC were harvest and injected via femoral artery to vascular foot bed. RESULT: No serious complication was observed during G-CSF injection, harvest and injection. Improvement in the ankle brachial pressure index was seen in all patients at 4 weeks and ischemic ulcer improved in 2 of 3 patients. Using of pain control decreased in all cases and increased blood flow at 99 m-Tc perfusion scan in 2 of 2 patients. CONCLUSION: These favorable outcomes strongly suggest a therapeutic impact of transplantation PBMNC in clinical setting.