Viability of Frozen-Thawed Embryos According to Developmental Stage in Mouse Embryo Cryopreservation / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 540-545, 2001.
Article
in Korean
| WPRIM
| ID: wpr-123579
ABSTRACT
OBJECT To compare the viability after thawing of cryopreserved embryos according to developmental stage and decide the cryopreservation strategy METHODS:
Total 538 mouse embryos(One, 2, 4, 8-cell, blastocyst, each 151, 136, 101, 98, 52, respectively) were cryopreserved and thawed using 1,2-propanediol and glycerol as cryoprotectant with slow cooling and rapid thawing technique, and compared the recovery, cleavage, blastocyst development rate, and calculated the recovery and reexpansion rate in blastocyst cryopreservation.RESULTS:
Highest recovery, cleavage and blastocyst development rate were obtained from one cell, 8-cell stage freezing, 90.1%, 89.5% and 76.7%, respectively. In recovery rate, there was no significant difference among developmental stage, but in cleavage rate, there was significant difference between 2 and 8-cell group(p<0.05). In blastocyst development rate, there was significant difference between 2 and 8 cell, 4 and 8-cell group(p<0.05). Recovery and reexpansion rate of frozen-thawed blastocyst was 73.1%, 52.6%, respectively.CONCLUSIONS:
Eight-cell embryo may be the best developmental stage for cryopreservation and blastocyst freezing also may be the promising technique.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blastocyst
/
Cryopreservation
/
Propylene Glycol
/
Embryonic Structures
/
Freezing
/
Glycerol
Limits:
Animals
Language:
Korean
Journal:
Korean Journal of Obstetrics and Gynecology
Year:
2001
Type:
Article
Similar
MEDLINE
...
LILACS
LIS