RFLP Analysis of a Gene for Blood Coagulation Factor IX in Korean / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 506-512, 2001.
Article
in Korean
| WPRIM
| ID: wpr-123584
ABSTRACT
OBJECTIVE:
The purpose of this study was to investigate the methods for analysis of restriction fragment length polymorphisms of hemophilia B (coagulation factorIX) gene in Korean population.METHODS:
Genomic DNAs were extracted from 40 Korean females. In order to amplify genomic DNAs at the region of the polymorphic sites, two sets of primers (Hha I and Dde I) were synthesized. The primers were named as FIX1, FIX2 for Hha I, and Dde I 59, Dde I 39 for Dde I, respectively. Hha I primers annealed 3'-flanking region of the FactorIX gene and amplified 230 bp long fragment. The PCR fragment (230 bp) treated with Hha I endonuclease produced two fragments (150 bp and 80 bp), when the polymorphic site existed. Dde I primers annealed the region of the first intron of Factor IX gene and amplified 319 bp long fragments. People cases with Dde I polymorphic site are supposed to produce 369 bp long fragment.Results:
It has been found that seven (14 X chromosomes) out of forty individuals showed Hha I polymorphism. However, none of the experimental People cases showed the Dde I polymorphism.CONCLUSIONS:
By the analysis of 80 chromosomes, the PICs calculated from allele frequency of Hha I-RFLP (0.175/0.825) and that of Dde I-RFLP (0.0/1.0) were 0.289=[1-(0.1752+0.8252)] and 0=[1-(02+12)], respectively. From these results, it can be postulated that Hha I and Dde I polymorphisms of the Factor IX gene in Korean exhibited different patterns from those of Caucasian.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood Coagulation
/
Polymorphism, Restriction Fragment Length
/
Blood Coagulation Factors
/
DNA
/
Factor IX
/
Introns
/
Polymerase Chain Reaction
/
Hemophilia B
/
Genes, vif
/
Dichlorodiphenyl Dichloroethylene
Limits:
Female
/
Humans
Language:
Korean
Journal:
Korean Journal of Obstetrics and Gynecology
Year:
2001
Type:
Article
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