Effects of Culture Condition on Secretion of Phospholipase from Candida albicans / 대한의진균학회지
Korean Journal of Medical Mycology
;
: 123-128, 1997.
Article
in Korean
| WPRIM
| ID: wpr-124247
ABSTRACT
BACKGROUND:
The dimorphic yeast, Candida albicans, is considered as a dangerous opportunistic pathogen in immunocompromised hosts. Several phospholipases of C. albicans are known to be secreted into the culture medium. Phospholipases have been proposed as a virulence factor in the pathogenesis of Candida infections.OBJECTIVE:
In order to investigate enzyme production, we examined culture condition of secreted phospholipase production from C. albicans.METHODS:
C. albicans ATCC 10231 was cultivated in various media at 37 degrees C for 3 days. Phospholipase activity was measured by fatty acid soap precipitation in plate containing 0.04% lecithin, 0.1 M citrate buffer, pH 4.2 and 1.5% noble agar.RESULTS:
Phospholipase was highly induced when C. albicans was cultivated in broth medium (containing glucose 2%, albumin 0.2% and Fe++ ion 0.01%) and Saboulaud's dextrose agar supplemented with 0.01% sodium deoxycholate.CONCLUSION:
Highly induction of secreted phospholipase by albumin from C albicans may be play an important role in tissue invasion in the pathogenesis of C. albicans.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phospholipases
/
Soaps
/
Virulence
/
Yeasts
/
Candida
/
Candida albicans
/
Immunocompromised Host
/
Citric Acid
/
Agar
/
Deoxycholic Acid
Language:
Korean
Journal:
Korean Journal of Medical Mycology
Year:
1997
Type:
Article
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