Mometasone Furoate Suppresses PMA-Induced MUC-5AC and MUC-2 Production in Human Airway Epithelial Cells / 결핵및호흡기질환
Tuberculosis and Respiratory Diseases
;
: 60-68, 2017.
Article
in English
| WPRIM
| ID: wpr-124432
ABSTRACT
BACKGROUND:
Mucus hypersecretion from airway epithelium is a characteristic feature of airway inflammatory diseases. Tumor necrosis factor α (TNF-α) regulates mucin synthesis. Glucocorticoids including mometasone fuorate (MF) have been used to attenuate airway inflammation. However, effects of MF on mucin production have not been reported.METHODS:
Effects of MF and budesonide (BUD) on the phorbol-12-myristate-13-acetate (PMA)–induction of mucin and TNF-α in human airway epithelial cells (NCI-H292) were investigated in the present study. Confluent NCI-H292 cells were pretreated with PMA (200 nM) for 2 hours. Subsequently, the cells were stimulated with MF (1–500 ng/mL) or BUD (21.5 ng/mL) for 8 hours. Dexamethasone (1 µg/mL) was used as the positive control. Real-time polymerase chain reaction was used to determine MUC2 and MUC5AC mRNA levels. The level of total mucin, MUC2, MUC5AC, and TNF-α in culture supernatants were measured using enzyme-linked immunosorbent assay.RESULTS:
MF and BUD significantly suppressed MUC2 and MUC5AC gene expression in PMA-stimulated NCI-H292 cells. The inhibitory effects of the two steroid drugs were also observed in the production of total mucin, MUC2 and MUC5AC proteins, and TNF-α.CONCLUSION:
Our findings demonstrated that MF and BUD attenuated mucin and TNF-α production in PMA-induced human airway epithelial cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Dexamethasone
/
Enzyme-Linked Immunosorbent Assay
/
Gene Expression
/
Tumor Necrosis Factor-alpha
/
Budesonide
/
Epithelial Cells
/
Epithelium
/
Real-Time Polymerase Chain Reaction
/
Mometasone Furoate
Limits:
Humans
Language:
English
Journal:
Tuberculosis and Respiratory Diseases
Year:
2017
Type:
Article
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