Apolipoprotein E Gene and Alpha 1-Antichymotrypsin Gene in Brain of Sporadic Alzheimer's Disease
Journal of the Korean Neurological Association
;
: 641-651, 2002.
Article
in Korean
| WPRIM
| ID: wpr-124508
ABSTRACT
BACKGROUND:
This study aims to detect any causative genetic alterations and to demonstrate any correlations of these genes in the pathogenesis of mostly late-occurring sporadic type of Alzheimer's disease (AD).METHODS:
A total of 67 registered cases of autopsy-confirmed brain tissues were analyzed. Included here was sporadic AD (n=41), vascular dementia (n=17), and non-demented physiologically aging control brains (n=9). ApoE genotyping was done with the enzymatic digestion, and allele specific PCR was done to analyze the -491 A/T polymorphism of ApoE. Detection of polymorphism of alpha 2-macroglobulin (A2M) was done with enzymatic digestion and DNA sequencing. RT-PCR products were electrophoresed to detect mRNA expression of alpha 1-antichymotrypsin (ACT).RESULTS:
A prevalence rate of ApoE E4 genotype (E3/E4, E4/E4) showed significantly higher in patients with AD than in patients with vascular dementia (43.8% vs. 11.7%, p=0.019). Only 1 out of 4 cases of sporadic AD was associated with the E4/E4 allele. -491A/ T polymorphism of the ApoE promoter was found only in AD (2/41 cases, 4.9%). The incidence of heterozygous allelic polymorphism with 5 bp deletions in exon 18 of A2M-2 was 4.9% (2 out of 41) in AD. Messenger RNA expression of ACT, which is closely associated with the ApoE E4 allele, was increased in AD in comparison with normal control (p=0.0002).CONCLUSIONS:
ApoE4 genotype and ACT are closely related to the pathogenesis of late-onset sporadic AD. Neither -491 polymorphism of ApoE promoter nor A2M-2 showed close association with AD in these brain samples.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Apolipoproteins
/
Apolipoproteins E
/
Brain
/
Aging
/
Alpha-Macroglobulins
/
RNA, Messenger
/
Dementia, Vascular
/
Alpha 1-Antichymotrypsin
/
Polymerase Chain Reaction
/
Exons
Type of study:
Incidence study
/
Prevalence study
/
Prognostic study
Limits:
Humans
Language:
Korean
Journal:
Journal of the Korean Neurological Association
Year:
2002
Type:
Article
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