A known expressed sequence tag, BM742401, is a potent lincRNA inhibiting cancer metastasis
Experimental & Molecular Medicine
;
: e31-2013.
Article
in English
| WPRIM
| ID: wpr-124615
ABSTRACT
Long intergenic non-coding RNAs (lincRNAs) have historically been ignored in cancer biology. However, thousands of lincRNAs have been identified in mammals using recently developed genomic tools, including microarray and high-throughput RNA sequencing (RNA-seq). Several of the lincRNAs identified have been well characterized for their functions in carcinogenesis. Here we performed RNA-seq experiments comparing gastric cancer with normal tissues to find differentially expressed transcripts in intergenic regions. By analyzing our own RNA-seq and public microarray data, we identified 31 transcripts, including a known expressed sequence tag, BM742401. BM742401 was downregulated in cancer, and its downregulation was associated with poor survival in gastric cancer patients. Ectopic overexpression of BM742401 inhibited metastasis-related phenotypes and decreased the concentration of extracellular MMP9. These results suggest that BM742401 is a potential lincRNA marker and therapeutic target.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phenotype
/
Stomach Neoplasms
/
RNA, Messenger
/
Gene Expression Regulation, Neoplastic
/
Proportional Hazards Models
/
Survival Analysis
/
Multivariate Analysis
/
Reproducibility of Results
/
Genetic Predisposition to Disease
/
Expressed Sequence Tags
Type of study:
Prognostic study
Limits:
Animals
/
Humans
/
Male
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2013
Type:
Article
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