Proteomic analysis of domestic pig pancreas during development using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry / 한국실험동물학회지
Laboratory Animal Research
;
: 45-53, 2014.
Article
in English
| WPRIM
| ID: wpr-124667
ABSTRACT
Pig pancreas may be a therapeutic resource for human diabetic patients. However, this potential is hindered by a lack of knowledge of the molecular events of pig pancreas development. In this study, the embryonic day 60, neonate and 6-month protein profiles of pig pancreas were ascertained at using two-dimensional gel electrophoresis and matrix assisted laser desorption/ionization-time of flight mass spectrometry. Twenty four proteins were differentially expressed during pig pancreas development. Among them, 12 spots increased and 7 spots decreased according to development. The expression of 5 protein were highest at birth. Expression of digestive enzymes including trypsin, pancreatic triacylglycerol lipase and pancreatic alpha-amylase was elevated in adults, whereas chymotrypsins were highly expressed in neonates. Proteins that were abundantly expressed during gestation were alpha-1-antitrypsin, alpha-fetoprotein and transferrins. Taken together, we found out that several proteins were significantly up- or down- regulated from pig pancreas based on developmental stage. This study will provide basis for understanding development of pig pancreas.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pancreas
/
Mass Spectrometry
/
Transferrin
/
Chymotrypsin
/
Trypsin
/
Electrophoresis, Gel, Two-Dimensional
/
Alpha-Fetoproteins
/
Sus scrofa
/
Parturition
/
Electrophoresis
Limits:
Adult
/
Humans
/
Infant, Newborn
/
Pregnancy
Language:
English
Journal:
Laboratory Animal Research
Year:
2014
Type:
Article
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