Two cases of partial-D showing different reactivity to various anti-D reagents / 대한수혈학회지
Korean Journal of Blood Transfusion
; : 55-59, 2003.
Article
in Ko
| WPRIM
| ID: wpr-125166
Responsible library:
WPRO
ABSTRACT
The 19-year-old twin sisters donated their blood in 2000. Their blood had typical Rh D negative red cell phenotype in Rh typing and weak D test using an anti-D reagent (Dade Behring, USA). Twin sisters donated blood again in 2001. Both were negative in anti-D reagent (Bioscotte Ltd., UK) and weakly positive in additively performed weak-D test. So we have acquired blood samples from them for further study in 2002. The red blood cells from twin sisters were not agglutinated with 4 various commercially available anti-D reagents. But in subsequently performed weak-D test, different reactivity to their anti-D reagents were shown, namely negative (Dade Behring, USA) and weakly positive (Ortho-clinical diagnostics, USA; Greencross, Korea; Bioscotte Ltd., UK). The lack of reactivity with some anti-D as shown in these cases can indicate the presence of a partial D antigen. So we carried out a additional serologic test using 6 monoclonal anti-D antibodies in partial-D typing set (Diamed, Switzerland) on Rh D antigens of red cells from twin sisters. According to the different reactivity patterns, we confirmed elder sister was partial-D category DFR and younger sister was partial-D with indeterminate category.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Phenotype
/
Twins
/
Serologic Tests
/
Siblings
/
Erythrocytes
/
Indicators and Reagents
/
Korea
/
Antibodies
Type of study:
Diagnostic_studies
Limits:
Humans
Country/Region as subject:
Asia
Language:
Ko
Journal:
Korean Journal of Blood Transfusion
Year:
2003
Type:
Article